The Effects of Induced Hypothyroidism on Plasma FSH and LH Concentrations in Female Wistar Rats

Thyroxin and triodothyroine are essential for normal organs growth development and function. These hormones regulate the basal metabolic rate (BMR) of all cells and play a critical role in the development of several organ systems such as reproductive. The experimental study was located at the University of Kwa Zulu-Natal (UKZN), Faculty of Science and Agriculture School of Biochemistry, Genetics and Microbiology, Westville campus South Africa, from October 2009 up to May 2010. Our objective is to investigate the effects of experimental induced hypothyroidism in females Wister rats FSH and LH concentrations and answer specific question what was worse to have low thyroid hormones in female Wistar rats. Carbmizole compound was administered orally to experimental animals. The dose was 50 mg/kg body weight for three weeks to induce the hypothyroid, euthyroid rats were received 3ml of deionized water. The concentrations of thyroid hormones (TSH, T3 and T4) and gonadtrophins (FSH, LH) were determined by using enzyme immunoassay kits from TOSOH, Corporation Japan. Through Hitach 906 analyzer. The results of body weight showed significant p ≤ 0.05 decreased in hypothyroid female rats group as compared to control group. TSH was showed significant p ≤ 0.05 decreased in hypothyroid rats as compared to control rats group, but T3 and T4 were showed decreased without significant. LH and FSH were showed conflict result FSH was decrease, but LH was increase without significant in hypothyroid compared to control rats group.